Immunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments
نویسندگان
چکیده
منابع مشابه
Immunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments.
T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a sy...
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Viral nucleoprotein complexes were extracted from the nuclei of simian virus 40 (SV40)-infected TC7 cells by low-salt treatment in the absence of detergent, followed by sedimentation on neutral sucrose gradients. Two forms of SV40 nucleoprotein complexes, those containing SV40 replicative intermediate DNA and those containing SV40 (I) DNA, were separated from one another and were found to have ...
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Tumor antigen (T-antigen) of simian virus 40 (SV40) has been shown to have a number of regulatory roles in both viral replication and early viral transcription. However, the nature of its role on late viral transcription remains unclear. We have analyzed for the presence of T-antigen on SV40 late viral transcription complexes which exhibit RNA polymerase II extension activity in vitro. Nuclear ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1976
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.17.2.668-671.1976